1×tbe buffer (Bio-Rad)

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Bio-Rad 1×tbe buffer
1×Tbe Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1%C3%97tbe+buffer/EDTA/us12436156-523-4-26
Average 96 stars, based on 1389 article reviews

1×tbe buffer - by Bioz Stars, 2026-09

96/100 stars

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Electrophoresis:

Article Title: DEVELOPMENT OF PROTOCOLS FOR THE CULTIVATION OF WORMWOOD ( ARTEMISIA SALSOLOIDES )
Article Snippet: .. The fragments were separated by electrophoresis in 1.5% agarose gel using 1×TBE buffer (Tris, Boric acid, EDTA) at a voltage of 80 V, 3 hours, power source − PowerPacBasic (BIO-RAD, USA). ..

Article Title: Programming DNA Nanoassemblies into Polyvalent Lysosomal Degraders for Potent Degradation of Pathogenic Membrane Proteins
Article Snippet: .. Following electrophoresis at 110 V for 1 h in 1×TBE buffer (89 mM Tris, 89 mM boric acid, 2.0 mM EDTA, pH 8.3), the gel was stained with GelRed and subsequently scanned and analyzed using the ChemiDoc Imaging System (BIO-RAD, USA). ..

Article Title: Programmable PCR-like Nonenzymatic DNA Molecular Circuit for Split-Free Autocatalytic Amplification
Article Snippet: .. Electrophoresis was performed at a constant potential of 120 V in 1×TBE buffer (89 mM Tris base, 89 mM Borate, 2 mM EDTA, pH=8.3) for 1.5 h. The gel was imaged by ChemiDocTM Imaging System (Bio-Rad, USA) under 365 nm UV irradiation after staining in diluted GelRedTM solution for 20 min. Serum sample collection and storage Blood samples were collected from patients attending the Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital prior to any treatment after informed consent was obtained. .. Whole blood was collected into 10 ml red topped clotting tubes (BD Biosciences).

Article Title: Construction of scalable multi-channel DNA nanoplatform for the combined detection of multi-component biomarkers of cancer
Article Snippet: .. The 12% PAGE was prepared, and electrophoresis was carried out in 1×TBE buffer (89×10− 3 M Tris-boric, 2×10− 3 M EDTA, pH = 8.0) at a constant voltage of 100 V for about 60 min. Gels imaging using a gel image system (Bio-Rad Laboratories). ..

Agarose Gel Electrophoresis:

Hood:

Article Title: Methods of determining pH and calcium or chloride concentration in samples
Article Snippet: .. Gels were run in 1×TBE buffer (100 mM Tris·HCl, 89 mM boric acid, and 2 mM EDTA, pH 8.3) at 4° C. Gels were observed under Biorad Universal Hood II Gel Doc System (Bio-Rad Laboratories, Inc.) using Image LabTM Software 6.0.0 for image acquisition for Alexa 647 and Alexa 546 channel. ..

Software:

Article Title: Asymmetric Hairpins DNA Encapsulated Silver Nanoclusters for In Situ Fluorescence Imaging of Fusion Gene Isoforms in Bone Marrow.
Article Snippet: Rapid and accurate imaging of the BCR/ABL fusion gene isoforms (e.g., e13a2, e14a2 and co-expression type) of chronic myeloid leukemia (CML) is of vital importance to first-line drug selection, but there is no assay that meets clinical needs (e.g., clinical kits > 18 h without isoforms information).. Herein, an in situ imaging platform is developed for the rapid and accurate detection of CML fusion gene isoforms using asymmetric sequence-enhanced hairpins DNA encapsulated silver nanoclusters (ADHA) and catalyzed hairpin assembly (CHA).. The specific detection of e13a2 and e14a2 fusion gene isoforms with detection limits of 19.2 am (11.558 copies μL−1) and 32.56 am (19.601 copies μL−1) in onepot is achieved.

Polyacrylamide Gel Electrophoresis:

Article Title: Simultaneous Visualization of Dual Intercellular Signal Transductions via SERS Imaging of Membrane Proteins Dimerization on Single Cells.
Article Snippet: .. S3 Instruments: The 10% native polyacrylamide gel electrophoresis (PAGE) was performed in 1×TBE buffer (80 mM Tris, 4 mM EDTA-2Na, 90 mM H3BO3, 12.5 mM MgCl2, pH 8.3) at room temperature by a Bio-Rad electrophoretic apparatus (PowerPac Basic, BIO-RAD, USA) operated at a constant voltage of 80 V for 80 min, and the electrophoretic patterns were imaged by a gel documentation system (Syngene, UK). .. Absorption spectra were scanned by a UV-Vis-NIR spectrophotometer (UV-3600, Shimadzu, Japan).

Fluorescence:

Sequencing:

Staining:

Imaging:

Irradiation:

Isolation:

Article Title: Structural features of T-DNA that induce transcriptional gene silencing during agroinfiltration
Article Snippet: .. Approximately 5 μg of total RNA isolated using the Trizol reagent was electrophoresed in 18% denaturing polyacrylamide gels containing 1×TBE buffer [89 mM Tris (pH 8.3), 89 mM boric acid, 2 mM EDTA] and 7 M urea, and blotted onto a nylon membrane (Zeta-Probe, Bio-Rad, USA) by electroblotting (1.0 mA/1.0 cm 2 membrane for 1 h at 4°C). .. DNA fragments of the GFP gene and the CaMV 35S promoter as probes were amplified by PCR, and then probes for siRNA detection were made using the BcaBEST Labeling Kit (Takara Bio) with [α- 32 P]dCTP.

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